Analysis of DNA intercalating drugs by TGGE

被引：5

作者：

Danko, P ^{[1
]}

Zozák, A ^{[1
]}

Podhradsky, D ^{[1
]}

Viglasky, V ^{[1
]}

机构：

[1] Safarik Univ, Fac Sci, Inst Chem, Dept Biochem, Kosice, Slovakia

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2005年 / 65卷 / 2-3期

关键词：

intercalators; daunomycin; doxorubicin; TGGE; temperature-gradient gel electrophoresis; scDNA; supercoiled DNA; ocDNA; open circular DNA;

D O I：

10.1016/j.jbbm.2005.10.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Temperature-gradient gel electrophoresis (TGGE) was used to study DNA-drug interactions. The results indicate that at least two classes of DNA intercalating drugs are distinguishable with respect to temperature increase: reversible and irreversible. The method offers an excellent means of visualizing the melting profile of an individual DNA topoisomer in the presence of DNA binding drugs. Our findings coincide with UV/VIS absorption spectroscopy data. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：89 / 95

页数：7

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