Circulating cell free DNA: Preanalytical considerations

被引：415

作者：

El Messaoudi, Safia ^{[1
,2
,3
,4
]}

Rolet, Fanny ^{[5
]}

Mouliere, Florent ^{[1
,2
,3
,4
,5
]}

Thierry, Alain R. ^{[1
,2
,3
,4
]}

机构：

[1] IRCM, F-34298 Montpellier, France

[2] INSERM, U896, F-34298 Montpellier, France

[3] Univ Montpellier I, F-34298 Montpellier, France

[4] Inst Reg Canc Montpellier, F-34298 Montpellier, France

[5] CNRS, SysDiag UMR3145, F-34184 Montpellier 4, France

来源：

CLINICA CHIMICA ACTA | 2013年 / 424卷

关键词：

Circulating cell-free DNA; Standard operating procedure; Preanalytical handling; Diagnosis; Stability; FREE PLASMA DNA; BLOOD-PROCESSING PROTOCOLS; FREE NUCLEIC-ACIDS; FREE FETAL DNA; ACQUIRED-RESISTANCE; MATERNAL PLASMA; CANCER-PATIENTS; TUMOR DNA; SERUM; QUANTIFICATION;

D O I：

10.1016/j.cca.2013.05.022

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Despite the growing interest in circulating cell-free DNA (ccfDNA) analysis in various clinical fields, especially oncology and prenatal diagnosis, few studies on sample handling have been reported and no analytical consensus is available. The lack of consistency between the various protocols for sample handling and the techniques used for ccfDNA analysis is one of the major obstacles in translating ccfDNA analysis to clinical practice. Although this point is highlighted regularly in the published reviews on ccfDNA analysis, no standard operating procedure currently exists despite several ongoing clinical studies on ccfDNA analysis. This review examines the preanalytical parameters potentially affecting ccfDNA concentration and fragmentation at each preanalytical step from blood drawing to the storage of ccfDNA extracts. Analysis of data in the literature and our own observations revealed the influence of preanalytical factors on ccfDNA analysis. Based on these data, we determined the optimal preanalytical protocols for ccfDNA analysis and ultimately, a guideline for the translation of ccfDNA analysis in routine clinical practice. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：222 / 230

页数：9

共 67 条

[1] Fetal cell-free plasma DNA concentrations in maternal blood are stable 24 hours after collection: Analysis of first- and third-trimester samples [J].