High performance liquid chromatography/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from Echinacea purpurea extracts

被引：74

作者：

Cech, NB ^{[1
]}

Eleazer, MS ^{[1
]}

Shoffner, LT ^{[1
]}

Crosswhite, MR ^{[1
]}

Davis, AC ^{[1
]}

Mortenson, AM ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem & Biochem, Greensboro, NC 27402 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1103卷 / 02期

关键词：

Echinacea purpurea; phenolics; alkamides; alkylamides; isobutylamides; caffeic acid derivatives; cichotic acid; caftaric acid; dodeca-2E; 4E; 8Z; 10Z-tetraenoic acid; HPLC; electrospray ionization mass spectrometry; ESI-MS; quadrupole ion trap;

D O I：

10.1016/j.chroma.2005.11.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracts of the plant Echinacea purpurea are widely used for medicinal purposes. Effective quality control of these extracts requires rapid methods to determine their chemical composition. A new method for analysis of caffeic acid derivatives and alkamides from Echinacea extracts has been developed. With this method, isomeric isobutylamides and 2-methylbutylamides can be distinguished, a capability that previously published methods have lacked. Quantitative analyses carried out with this method on E. purpurea extracts that have been stored for 18 months indicate that they contain caftaric acid, cichoric acid, and undeca-2Z,4E-diene-8, 10-diynoic acid isobutylamide at concentrations of 0.7, 0.71 and 2.0 mg/mL, respectively. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：219 / 228

页数：10

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