Ultra performance liquid chromatography (UPLC) further improves hydrogen/deuterium exchange mass spectrometry

被引：55

作者：

Wu, Y

Engen, JR

Hobbins, WB

机构：

[1] Univ New Mexico, Dept Chem, Albuquerque, NM 87131 USA

[2] Waters Corp, Milford, MA USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2006年 / 17卷 / 02期

关键词：

D O I：

10.1016/j.jasms.2005.10.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ultra performance liquid chromatography (UPLC) employs particles smaller than 2 Am in diameter to achieve superior resolution, speed, and sensitivity compared with high-performance liquid chromatography (HPLC). We have tested the suitability of UPLC for the analysis of deuterated peptides in hydrogen exchange mass spectrometry experiments. Superior resolution and sample throughput were obtained with UPLC versus HPLC. For highly deuterated model peptides, deuterium loss using UPLC was greater than the deuterium loss observed using a conventional HPLC system, primarily as a result of the injection requirements of the UPLC system. Partially deuterated cytochrome c peptides also lost more deuterium in UPLC versus HPLC, although the effect was not as pronounced as it was for the highly deuterated model peptides. The exceptional chromatographic aspects of UPLC make it a very attractive alternative to HPLC for hydrogen exchange mass spectrometry experiments.

引用

页码：163 / 167

页数：5

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