Simultaneous quantification of organic solvent biomarkers by high performance liquid chromatography

被引：9

作者：

Bulcao, Rachel ^{[1
]}

Maria, Lucas Santa ^{[1
]}

Charao, Mariele ^{[1
]}

Moro, Angela ^{[1
]}

Roehrs, Miguel ^{[1
]}

Garcia, Solange Cristina ^{[1
]}

Limberger, Renata Pereira ^{[2
]}

机构：

[1] Univ Fed Santa Maria, Dept Anal Clin & Toxicol, BR-97110970 Santa Maria, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Fac Farm, Dept Anal Clin & Toxicol, BR-90610000 Porto Alegre, RS, Brazil

来源：

QUIMICA NOVA | 2008年 / 31卷 / 06期

关键词：

HPLC; organic solvents; biological exposure index;

D O I：

10.1590/S0100-40422008000600013

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Biological monitoring is very important to guarantee health to workers. This method was developed for Simultaneous determination of xylene, toluene, styrene and ethylbenzene metabolites. It involves only dilution and centrifugation Of Urine samples and improved chromatographic conditions. Analyses show recovery >= 95%; r(2) > 0.99; intermediate precision CV% < 6% and % bias < 10. Exposed subjects presented at least three metabolites in urine. The method proved to be feasible, reliable and important in biological monitoring, especially in exposure to organic solvent mixtures.

引用

页码：1343 / 1348

页数：6

共 29 条

[11] Biological monitoring for exposure to volatile organic compounds (VOCs) [J].

Heinrich-Ramm, R ;

Jakubowski, M ;

Heinzow, B ;

Christensen, JM ;

Olsen, E ;

Hertel, O .

PURE AND APPLIED CHEMISTRY, 2000, 72 (03) :385-436

[12]

ICH, 1996, ICH Q2B VAL AN PROC

[13]

JOHNSON AC, 1995, OCCUP MED, V10, P623

[14]

Kirk R. E., 1984, ENCY CHEM TECHNOLOGY

[15]

KLAASSEN CD, 2001, OCCUPATIONAL TOXICOL

[16] Modified method for determination of hippuric acid and methylhippuric acid in urine by gas chromatography [J].

Kongtip, P ;

Vararussami, J ;

Pruktharathikul, V .

JOURNAL OF CHROMATOGRAPHY B, 2001, 751 (01) :199-203

[17]

LAFFON B, 2001, LIFE SCI, V753, P385

[18]

LAUWERYS RR, 1996, OCCUPATIONAL TOXICOL

[19] Liquid chromatography-mass spectrometry in occupational toxicology: A novel approach to the study of biotransformation of industrial chemicals [J].

Manini, P ;

Andreoli, R ;

Niessen, WMA .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1058 (1-2) :21-37

[20]

MCDIARMID MA, 1995, PATOLOGIA TRABALHO

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