Poly(N,N-dimethylacrylamide)-Coated Maghemite Nanoparticles for Stem Cell Labeling

被引:80
作者
Babic, Michal [1 ,2 ]
Horak, Daniel [1 ,2 ]
Jendelova, Pavla [2 ,3 ]
Glogarova, Katerina [3 ]
Herynek, Vit [2 ,4 ]
Trchova, Miroslava [1 ]
Likavcanova, Katarina [3 ]
Lesny, Petr [2 ,3 ]
Pollert, Emil [5 ]
Hajek, Milan [2 ,4 ]
Sykova, Eva [2 ,3 ]
机构
[1] Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague 6, Czech Republic
[2] Charles Univ Prague, Ctr Cell Therapy & Tissue Repair, Prague 15006 5, Czech Republic
[3] Acad Sci Czech Republ, Inst Expt Med, Prague 14220 4, Czech Republic
[4] Inst Clin & Expt Med, Prague 14021 4, Czech Republic
[5] Acad Sci Czech Republ, Inst Phys, Prague 16253 6, Czech Republic
关键词
IRON-OXIDE NANOPARTICLES; IN-VIVO; SUPERPARAMAGNETIC NANOPARTICLES; MIGRATION; PROGENITOR; PARTICLES; TRACKING; AGENT;
D O I
10.1021/bc800373x
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Maghemite (gamma-Fe2O3) nanoparticles were obtained by the coprecipitation of Fe(II) and Fe (III) salts with ammonium hydroxide followed by oxidation with sodium hypochlorite. Solution radical polymerization of N,N-dimethylacrylamide (DMAAm) in the presence of maghemite nanoparticles yielded poly(N,N-dimethylacrylamide) (PDMAAm)-coated maghemite nanoparticles. The presence of PDMAAm on the maghemite particle surface was confirmed by elemental analysis and ATR FTIR spectroscopy. Other methods of nanoparticle characterization involved scanning and transmission electron microscopy, atomic adsorption spectroscopy (AAS), and dynamic light scattering (DLS). The conversion of DMAAm during polymerization and the molecular weight of PDMAAm bound to maghemite were determined by using gas and size-exclusion chromatography, respectively. The effect of ionic 4,4'-azobis(4-cyanovaleric acid) (ACVA) initiator on nanoparticle morphology was elucidated. The nanoparticles exhibited long-term colloidal stability in water or physiological buffer. Rat and human bone marrow mesenchymal stem cells (MSCs) were labeled with uncoated and PDMAAm-coated maghemite nanoparticles and with Endorem as a control. Uptake of the nanoparticles was evaluated by Prussian Blue staining, transmission electron microscopy, T-2-MR relaxometry, and iron content analysis. Significant differences in labeling efficiency were found for human and rat cells. PDMAAm-modified nanoparticles demonstrated a higher efficiency of intracellular uptake into human cells in comparison with that of dextran-modified (Endorem) and unmodified nanoparticles. In gelatin, even a small number of labeled cells changed the contrast in MR images. PDMAAm-coated nanoparticles provided the highest T-2 relaxivity of all the investigated particles. In vivo MR imaging of PDMAAm-modified iron oxide-labeled rMSCs implanted in a rat brain confirmed their better resolution compared with Endorem-labeled cells.
引用
收藏
页码:283 / 294
页数:12
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