Surface modification to reduce nonspecific binding of quantum dots in live cell assays

被引：222

作者：

Bentzen, EL

Tomlinson, ID

Mason, J

Gresch, P

Warnement, MR

Wright, D

Sanders-Bush, E

Blakely, R

Rosenthal, SJ

机构：

[1] Vanderbilt Univ, Dept Chem, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN USA

来源：

BIOCONJUGATE CHEMISTRY | 2005年 / 16卷 / 06期

关键词：

D O I：

10.1021/bc0502006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonspecific binding is a frequently encountered problem with fluorescent labeling of tissue cultures when labeled with quantum dots. In these studies various cell lines were examined for nonspecific binding. Evidence suggests that nonspecific binding is related to cell type and may be significantly reduced by functionalizing quantum dots with poly(ethylene glycol) ligands (PEG). The length of PEG required to give a significant reduction in nonspecific binding may be as short as 12-14 ethylene glycol units.

引用

页码：1488 / 1494

页数：7

共 29 条

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