Colorimetric biosensing of pathogens using gold nanoparticles

被引:144
作者
Verma, Mohit S. [1 ,2 ]
Rogowski, Jacob L. [1 ,2 ]
Jones, Lyndon [1 ,3 ]
Gu, Frank X. [1 ,2 ]
机构
[1] Univ Waterloo, Dept Chem Engn, Waterloo, ON N2L 3G1, Canada
[2] Univ Waterloo, Waterloo Inst Nanotechnol, Waterloo, ON N2L 3G1, Canada
[3] Univ Waterloo, Ctr Contact Lens Res, Waterloo, ON N2L 3G1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Bacteria; Virus; Fungus; Point-of-care; DNA; RNA; Protein; Small molecule; PCR; Color; SURFACE-PLASMON RESONANCE; POLYMERASE-CHAIN-REACTION; SEQUENCE-BASED AMPLIFICATION; ESCHERICHIA-COLI O157H7; GENOMIC DNA; STAPHYLOCOCCUS-AUREUS; CAMPYLOBACTER-JEJUNI; OPTICAL-DETECTION; AU NANOPARTICLES; INFLUENZA-VIRUS;
D O I
10.1016/j.biotechadv.2015.03.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Rapid detection of pathogens is crucial to minimize adverse health impacts of nosocomial, foodborne, and water-borne diseases. Gold nanoparticles are extremely successful at detecting pathogens due to their ability to provide a simple and rapid color change when their environment is altered. Here, we review general strategies of implementing gold nanoparticles in calorimetric biosensors. First, we highlight how gold nanoparticles have improved conventional genomic analysis methods by lowering detection limits while reducing assay times. Then, we focus on emerging point-of-care technologies that aim at pathogen detection using simpler assays. These advances will facilitate the implementation of gold nanoparticle-based biosensors in diverse environments throughout the world and help prevent the spread of infectious diseases. (C) 2015 Elsevier Inc All rights reserved.
引用
收藏
页码:666 / 680
页数:15
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