Electrophoretic and immunoblot analysis of Campylobacter fetus lipopolysaccharides

被引:6
作者
Brooks, BW [1 ]
Garcia, MM [1 ]
Robertson, RH [1 ]
Lior, H [1 ]
机构
[1] LAB CTR DIS CONTROL,NATL REFERENCE SERV CAMPYLOBACTERS,OTTAWA,ON K1A 0L2,CANADA
关键词
Campylobacter fetus; lipopolysaccharide; SDS-PAGE; immunoblot;
D O I
10.1016/0378-1135(96)00015-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Proteinase K-digested cell lysates from 25 Campylobacter fetus subspecies fetus and C. fetus subsp. venerealis strains were examined by SDS-PAGE and immunoblotting. Three SDS-PAGE lipopolysaccharide (LPS) profiles were observed. Two profiles were consistent with those previously reported for serogroup A and serogroup B and AB isolates and were distinguished by the relative mobility of bands in the O-chain region and by a strong reaction on immunoblots with homologous antisera. The third profile was similar but had faster migrating O-chain bands. Immunoblot reactions using homologous and heterologous adsorbed antisera showed that the O-antigen of the C. fetus subsp. fetus reference strain and other profile 2-type LPS strains was distinct from the O-antigens of strains with profile 1- or profile 3-type LPS. O-antigens of strains with profile 1- and profile 3-type LPS had shared epitopes. One strain had core components but no detectable O-antigens. Common core LPS antigens appear to be present in all strains and antibodies to common core LPS epitopes may be useful reagents for rapid detection of C. fetus.
引用
收藏
页码:105 / 114
页数:10
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