Electrophoretic and immunoblot analysis of Campylobacter fetus lipopolysaccharides

被引：6

作者：

Brooks, BW ^{[1
]}

Garcia, MM ^{[1
]}

Robertson, RH ^{[1
]}

Lior, H ^{[1
]}

机构：

[1] LAB CTR DIS CONTROL,NATL REFERENCE SERV CAMPYLOBACTERS,OTTAWA,ON K1A 0L2,CANADA

来源：

VETERINARY MICROBIOLOGY | 1996年 / 51卷 / 1-2期

关键词：

Campylobacter fetus; lipopolysaccharide; SDS-PAGE; immunoblot;

D O I：

10.1016/0378-1135(96)00015-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Proteinase K-digested cell lysates from 25 Campylobacter fetus subspecies fetus and C. fetus subsp. venerealis strains were examined by SDS-PAGE and immunoblotting. Three SDS-PAGE lipopolysaccharide (LPS) profiles were observed. Two profiles were consistent with those previously reported for serogroup A and serogroup B and AB isolates and were distinguished by the relative mobility of bands in the O-chain region and by a strong reaction on immunoblots with homologous antisera. The third profile was similar but had faster migrating O-chain bands. Immunoblot reactions using homologous and heterologous adsorbed antisera showed that the O-antigen of the C. fetus subsp. fetus reference strain and other profile 2-type LPS strains was distinct from the O-antigens of strains with profile 1- or profile 3-type LPS. O-antigens of strains with profile 1- and profile 3-type LPS had shared epitopes. One strain had core components but no detectable O-antigens. Common core LPS antigens appear to be present in all strains and antibodies to common core LPS epitopes may be useful reagents for rapid detection of C. fetus.

引用

页码：105 / 114

页数：10

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