Analysis of oligonucleotides by on-column transient capillary isotachophoresis and capillary electrophoresis in poly(ethylene glycol)-filled columns

被引：49

作者：

Auriola, S ^{[1
]}

Jaaskelainen, I ^{[1
]}

Regina, M ^{[1
]}

Urtti, A ^{[1
]}

机构：

[1] UNIV KUOPIO,DEPT PHARMACEUT CHEM,SF-70211 KUOPIO,FINLAND

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 22期

关键词：

D O I：

10.1021/ac960872c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis in poly(ethylene glycol) (PEG) solution-filled uncoated silica capillaries was demonstrated to be suitable for separation of oligonucleotides in 100 mM ammonium formate (pH 4.5) buffers. Reversed polarity (-30 kV at the injector end) was used, because the electroosmotic now (EOF) at 4.5 was considerably lower than the mobility of the anionic analytes. On-column transient isotachophoresis (ITP) was developed for preconcentration of oligonucleotide samples in PEG solution-filled capillaries, The ITP focusing step allows injection of 20% of column length without loss of peak resolution. The detection limit of the method was 100 ng/mL of 16-mer oligonucleotide by using UV detection at wavelength 254 nm (signal-to-noise ratio 10).

引用

页码：3907 / 3911

页数：5

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