Extraction of Candida antarctica lipase A from aqueous solutions using imidazolium-based ionic liquids

被引：50

作者：

Deive, Francisco J. ^{[1
,2
]}

Rodriguez, Ana ^{[1
,2
]}

Rebelo, Luis P. N. ^{[1
]}

Marrucho, Isabel M. ^{[1
,3
]}

机构：

[1] UNL, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal

[2] Univ Vigo, Dept Ingn Quim, Vigo 36310, Spain

[3] Univ Aveiro, Dept Quim, P-3810193 Aveiro, Portugal

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2012年 / 97卷

关键词：

Ionic liquids; Aqueous biphasic system; Green biotechnology; Extraction; Lipase; 2-PHASE EXTRACTION; BIPHASIC SYSTEMS; BIOCATALYSIS; PROTEINS; STABILIZATION; DYNAMICS; ENZYMES; DESIGN; WATER;

D O I：

10.1016/j.seppur.2011.12.013

中图分类号：

TQ [化学工业];

学科分类号：

081705 [工业催化];

摘要：

The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge-density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data. (c) 2011 Elsevier B.V. All rights reserved.

引用

页码：205 / 210

页数：6

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