Ultrafast fluorescence spectroscopy of biologically relevant chromophores using type II difference frequency generation

被引:7
作者
Leonard, J. [1 ]
Gelot, T. [1 ]
Torgasin, K. [1 ]
Haacke, S. [1 ]
机构
[1] Strasbourg Univ, Inst Phys & Chim Mat Strasbourg, Dept Ultrafast Opt & Nanophoton, CNRS,UMR 7504, F-67034 Strasbourg, France
来源
9TH INTERNATIONAL CONFERENCE ON PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE (PIBM 2010) | 2011年 / 277卷
关键词
UP-CONVERSION; LUMINESCENCE SPECTROSCOPY; KERR SHUTTER; DYNAMICS; RECOGNITION; RESOLUTION; COMPLEX; STATE; GATE;
D O I
10.1088/1742-6596/277/1/012017
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
A novel femtosecond fluorescence experiment based on type II difference frequency mixing is demonstrated. This approach is particularly interesting for near-UV emitting biological chromophores like amino acids and nucleotides, as the fluorescence is converted into the spectral range where CCD have their highest quantum efficiencies. The method is implemented with a 5-kHz amplified Ti:Sapphire laser system and first results obtained with 2,5-diphenyloxazole (PPO) in ethanol are reported.
引用
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页数:5
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