Ultrafast fluorescence spectroscopy of biologically relevant chromophores using type II difference frequency generation

被引:7
作者
Leonard, J. [1 ]
Gelot, T. [1 ]
Torgasin, K. [1 ]
Haacke, S. [1 ]
机构
[1] Strasbourg Univ, Inst Phys & Chim Mat Strasbourg, Dept Ultrafast Opt & Nanophoton, CNRS,UMR 7504, F-67034 Strasbourg, France
来源
9TH INTERNATIONAL CONFERENCE ON PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE (PIBM 2010) | 2011年 / 277卷
关键词
UP-CONVERSION; LUMINESCENCE SPECTROSCOPY; KERR SHUTTER; DYNAMICS; RECOGNITION; RESOLUTION; COMPLEX; STATE; GATE;
D O I
10.1088/1742-6596/277/1/012017
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
A novel femtosecond fluorescence experiment based on type II difference frequency mixing is demonstrated. This approach is particularly interesting for near-UV emitting biological chromophores like amino acids and nucleotides, as the fluorescence is converted into the spectral range where CCD have their highest quantum efficiencies. The method is implemented with a 5-kHz amplified Ti:Sapphire laser system and first results obtained with 2,5-diphenyloxazole (PPO) in ethanol are reported.
引用
收藏
页数:5
相关论文
共 17 条
[11]   Broad-band fluorescence upconversion for femtosecond spectroscopy [J].
Schanz, R ;
Kovalenko, SA ;
Kharlanov, V ;
Ernsting, NP .
APPLIED PHYSICS LETTERS, 2001, 79 (05) :566-568
[12]   A broadband Kerr shutter for femtosecond fluorescence spectroscopy [J].
Schmidt, B ;
Laimgruber, S ;
Zinth, W ;
Gilch, P .
APPLIED PHYSICS B-LASERS AND OPTICS, 2003, 76 (08) :809-814
[13]   ULTRAFAST LUMINESCENCE SPECTROSCOPY USING SUM FREQUENCY GENERATION [J].
SHAH, J .
IEEE JOURNAL OF QUANTUM ELECTRONICS, 1988, 24 (02) :276-288
[14]   RNA-protein recognition: Single-residue ultrafast dynamical control of structural specificity and function [J].
Xia, TB ;
Wan, CZ ;
Roberts, RW ;
Zewail, AH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13013-13018
[15]   The RNA-protein complex: Direct probing of the interfacial recognition dynamics and its correlation with biological functions [J].
Xia, TB ;
Becker, HC ;
Wan, CZ ;
Frankel, A ;
Roberts, RW ;
Zewail, AH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (14) :8119-8123
[16]   Ultrafast excited state dynamics of the protonated Schiff base of all-trans retinal in solvents [J].
Zgrablic, G ;
Voïtchovsky, K ;
Kindermann, M ;
Haacke, S ;
Chergui, M .
BIOPHYSICAL JOURNAL, 2005, 88 (04) :2779-2788
[17]   Femtosecond fluorescence spectroscopy by upconversion with tilted gate pulses [J].
Zhao, LJ ;
Lustres, JLP ;
Farztdinov, V ;
Ernsting, NP .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2005, 7 (08) :1716-1725