Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues

被引：49

作者：

Posa, Francesca ^{[1
]}

Di Benedetto, Adriana ^{[1
]}

Colaianni, Graziana ^{[2
]}

Cavalcanti-Adam, Elisabetta A. ^{[3
,4
]}

Brunetti, Giacomina ^{[2
]}

Porro, Chiara ^{[1
]}

Trotta, Teresa ^{[1
]}

Grano, Maria ^{[2
]}

Mori, Giorgio ^{[1
]}

机构：

[1] Univ Foggia, Sch Med, Dept Clin & Expt Med, Foggia, Italy

[2] Univ Bari, Sect Human Anat & Histol, Dept Basic & Med Sci Neurosci & Sense Organs, Bari, Italy

[3] Heidelberg Univ, Inst Phys Chem, Dept Biophys Chem, Heidelberg, Germany

[4] Max Planck Inst Intelligent Syst, Stuttgart, Germany

来源：

STEM CELLS INTERNATIONAL | 2016年 / 2016卷

关键词：

OSTEOGENIC DIFFERENTIATION; IN-VITRO; BONE SIALOPROTEIN; D-RECEPTOR; SECRETED PHOSPHOPROTEIN-1; STROMAL CELLS; GOOD PROMISE; D DEFICIENCY; PULP; EXPRESSION;

D O I：

10.1155/2016/9150819

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

1 alpha,25-Dihydroxyvitamin D-3 (1,25(OH)(2) D-3), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of human Mesenchymal Stem Cells (hMSCs) from bone marrow. MSCs can be also isolated from the immature form of the tooth, the dental bud: Dental Bud Stem Cells (DBSCs) are adult stem cells that can effectively undergo osteoblastic differentiation. In this work we investigated the effect of Vit D on DBSCs differentiation into osteoblasts. Our data demonstrate that DBSCs, cultured in an opportune osteogenic medium, differentiate into osteoblast-like cells; Vit D treatment stimulates their osteoblastic features, increasing the expression of typical markers of osteoblastogenesis like RUNX2 and Collagen I (Coll I) and, in a more important way, determining a higher production of mineralized matrix nodules.

引用

页数：9

共 52 条

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In vitro stem cell cultures from human dental pulp and periodontal ligament:: New prospects in dentistry [J].