Development and evaluation of a multiplex PCR for eight plasmid-mediated quinolone-resistance determinants

被引：134

作者：

Ciesielczuk, H. ^{[1
,2
]}

Hornsey, M. ^{[1
,3
]}

Choi, V. ^{[1
,4
]}

Woodford, N. ^{[1
,2
]}

Wareham, D. W. ^{[1
]}

机构：

[1] Univ London, Ctr Immunol & Infect Dis, Barts & London Sch Med & Dent, Blizard Inst,Antimicrobial Res Grp, London E1 2AT, England

[2] Publ Hlth England, Antimicrobial Resistance & Healthcare Associated, London NW9 5EQ, England

[3] Univ Roehampton, Whitelands Coll, Dept Life Sci, London SW15 4JD, England

[4] Singapore Gen Hosp, Singapore 169037, Singapore

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2013年 / 62卷

关键词：

ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; SALMONELLA-ENTERICA; EXTENDED-SPECTRUM; BETA-LACTAMASES; GENE; PREVALENCE; ANIMALS; STRAINS;

D O I：

10.1099/jmm.0.064428-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

The objective of this study was to develop and validate an expanded multiplex PCR assay for the simultaneous detection of eight plasmid-mediated quinolone-resistance determinants in Enterobacteriaceae. Primers were designed to amplify conserved fragments of qnrABCDS, qepA, oqxAB and aac(6')-lb-cr genes and were optimized in uniplex and multiplex PCR assays with control template DNA. The assay was used to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in 174 ciprofloxacin-resistant and 43 ciprofloxacin-susceptible extraintestinal pathogenic Escherichia coli isolates. Each resistance gene could be detected alone and in combination. PMQR determinants were detected in 65 ciprofloxacin-resistant isolates (37%) and one ciprofloxacin-susceptible isolate (2%). Prevalences of the identified determinants were: aac(6')-lb-cr, 34.5 %; qnrS, 1.1 Wo; qepA, 1.1 %; and oqxAB, 0.6%. In conclusion, we developed an eight-target multiplex PCR for the accurate detection of PMQR genes and confirmed that PMQR prevalence remains low among human Escherichia coli clinical isolates in the UK.

引用

页码：1823 / 1827

页数：5

共 31 条

[1]

Plasmid-mediated quinolone resistance genes in Enterobacteriaceae isolates associated with community and nosocomial urinary tract infection in East London, UK [J].