Population structure, virulence potential and antibiotic susceptibility of uropathogenic Escherichia coli from Northwest England

被引:143
作者
Gibreel, Tarek M. [1 ]
Dodgson, Andrew R. [1 ,2 ]
Cheesbrough, John [3 ]
Fox, Andrew J. [4 ]
Bolton, Frederick J. [5 ]
Upton, Mathew [1 ]
机构
[1] Univ Manchester, Sch Translat Med, Microbiol & Virol Unit, Manchester M13 9WL, Lancs, England
[2] Cent Manchester Fdn Trust, Manchester M13 9WL, Lancs, England
[3] Lancashire Teaching Hosp NHS Fdn Trust, Royal Preston Hosp, Preston Microbiol Serv, Preston PR2 9HT, Lancs, England
[4] Royal Preston Hosp, Hlth Protect Agcy, Food Water & Environm Microbiol Lab, Preston PR2 9HT, Lancs, England
[5] Manchester Royal Infirm, Hlth Protect Agcy, North West Lab, Manchester M13 9WZ, Lancs, England
关键词
UPEC; MLST; population genetics; ESBL; SPECTRUM BETA-LACTAMASES; RESISTANCE QNR GENES; URINARY-TRACT; CLONAL GROUPS; CIPROFLOXACIN-RESISTANT; MOLECULAR EPIDEMIOLOGY; SEQUENCE; STRAINS; QUINOLONE; ST131;
D O I
10.1093/jac/dkr451
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: Multilocus sequence typing (MLST) has been used to characterize diverse pathogens, including uropathogenic Escherichia coli (UPEC). There has been significant interest in the contribution of the O25b: H4-ST131 lineage to UPEC disease, as these isolates are often highly virulent and exhibit multidrug resistance. To reveal the wider impact of sequence type (ST) 131, we have examined its contribution to the overall population structure of UPEC isolates that were not selected on the basis of virulence or antibiotic resistance. Methods: Three hundred UPEC isolates were recovered from community and hospital urine samples examined by clinical microbiology laboratories in the Northwest region of England in June 2007 and June 2009. They were characterized by susceptibility profiling, MLST and virulence gene PCR. PFGE was used to examine isolates from key clones. Results: The most common lineage was ST73 (16.6%) followed by ST131 (13.3%), ST69 (9%), ST95 (6.3%), ST10 (4.3%) and ST127 (3.6%). ST131 isolates were significantly more likely to exhibit high levels of antibiotic resistance (35% being CTX-M-15 PCR positive) and those of ST127 were the most widely susceptible but carried the highest number of virulence genes. Only when the CTX-M-15-O25b-positive strains were examined was a high level of virulence observed for ST131 isolates. PFGE indicated ongoing local evolution in ST131. Conclusions: ST131 isolates are well established in the wider UPEC population. This clone is still evolving and we further support suggestions that it represents a real threat to health. We suggest that ST127 is a recently emerged, community-associated, virulent clone that warrants further study.
引用
收藏
页码:346 / 356
页数:11
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