Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

被引：1458

作者：

Kostic, Aleksandar D. ^{[1
,2
]}

Gevers, Dirk ^{[1
]}

Pedamallu, Chandra Sekhar ^{[1
,3
]}

Michaud, Monia ^{[4
]}

Duke, Fujiko ^{[1
,3
]}

Earl, Ashlee M. ^{[1
]}

Ojesina, Akinyemi I. ^{[1
,3
]}

Jung, Joonil ^{[1
]}

Bass, Adam J. ^{[1
,3
]}

Tabernero, Josep ^{[5
]}

Baselga, Jose ^{[5
]}

Liu, Chen ^{[6
,7
,8
]}

Shivdasani, Ramesh A. ^{[3
]}

Ogino, Shuji ^{[2
,3
,9
]}

Birren, Bruce W. ^{[1
]}

Huttenhower, Curtis ^{[1
,10
]}

Garrett, Wendy S. ^{[1
,3
,4
]}

Meyerson, Matthew ^{[1
,2
,3
]}

机构：

[1] Broad Inst Massachusetts Inst Technol & Harvard, Cambridge, MA 02142 USA

[2] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[3] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[4] Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA

[5] Univ Autonoma Barcelona, Dept Med Oncol, Vall dHebron Univ Hosp, Barcelona 08035, Spain

[6] Univ Florida, Coll Med, Dept Pathol, Gainesville, FL 32610 USA

[7] Univ Florida, Coll Med, Dept Immunol, Gainesville, FL 32610 USA

[8] Univ Florida, Coll Med, Dept Lab Med, Gainesville, FL 32610 USA

[9] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA

[10] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA

来源：

GENOME RESEARCH | 2012年 / 22卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

HELICOBACTER-PYLORI; BACTERIA; RESOURCE;

D O I：

10.1101/gr.126573.111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.

引用

页码：292 / 298

页数：7

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