The membrane-bound electron transport system of Methanosarcina species

Members of the genus Methanosarcina are strictly anaerobic archaea that derive their metabolic energy from the conversion of a restricted number of substrates to methane. H-2 + CO2 are converted to CH4 via the CO2-reducing pathway, while methanol and methylamines are metabolized by the methylotrophic pathway. Two novel electron transport systems are involved in the process of methanogenesis. Both systems are able to use a heterodisulfide as electron acceptor and either H-2 or F420H2 as electron acceptors and generate a proton-motive force by redox potential-driven H+-translocation. The H-2: heterodisulfide oxidoreductase is composed of an F-420-nonreducing hydrogenase and the heterodisulfide reductase. The latter protein is also part of the F420H2: heterodisulfide oxidoreductase system. The second component of this system is referred to as F420H2 dehydrogenase. The archaeal protein is a homologue of complex I of the respiratory chain from bacteria and mitochondria. This review focuses on the biochemical and genetic characteristics of the three energy-transducing enzymes and on the mechanisms of ion translocation.