Anti-human red cell monoclonal antibodies produced by macaque-mouse heterohybridomas: Their reactivity with human and nonhuman primate erythrocytes

被引：4

作者：

Blancher, A ^{[1
]}

Roubinet, F

Reld, M

Socha, WW

机构：

[1] CHU Purpan, Immunol Lab, F-31059 Toulouse, France

[2] Etab Transfus Sanguine Pyrenees Garonne, Lab Immunohematol, F-31027 Toulouse, France

[3] New York Blood Ctr, New York, NY 10021 USA

[4] NYU, Med Ctr, Lab Expt Med & Surg Primate, New York, NY 10016 USA

来源：

JOURNAL OF MEDICAL PRIMATOLOGY | 1999年 / 28卷 / 03期

关键词：

blood group antigens; rhesus monkey; cell fusion;

D O I：

10.1111/j.1600-0684.1999.tb00259.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Eighteen monoclonal antibodies (Mabs) against human red blood cells (RBCs) produced by macaque-mouse heterobybridomas gave uniformly positive reactions with all human samples except for some with particular null phenotypes. Based on reactions with latter cells, the human antigenic targets of 11 antibodies could be identified: six were specific for glycophorin-related antigens (Wr(b), En(a), Ge4), and each of the five remaining antibodies showed one of the following specificities: CD55, CD44, CD59, Kell, and Rh proteins. Four Mabs recognized the V-c antigen of the chimpanzee V-A-B-D system. Six macaque Mabs detected polymorphisms in chimpanzee, gorilla, orangutan, and gibbon that did not correspond to any known blood group in these animals, while other Mabs gave monomorphic reactions with ape RBCs. The reagents produced by macaque hybridomas are useful tools not only for human blood grouping tests, but also for tracing the relationships among blood group antigens of man and anthropoid apes.

引用

页码：118 / 128

页数：11