LincRNA-p21 Suppresses Target mRNA Translation

被引：1360

作者：

Yoon, Je-Hyun ^{[1
]}

Abdelmohsen, Kotb ^{[1
]}

Srikantan, Subramanya ^{[1
]}

Yang, Xiaoling ^{[1
]}

Martindale, Jennifer L. ^{[1
]}

De, Supriyo ^{[2
]}

Huarte, Maite ^{[3
]}

Zhan, Ming ^{[4
]}

Becker, Kevin G. ^{[2
]}

Gorospe, Myriam ^{[1
]}

机构：

[1] NIA, Lab Mol Biol & Immunol, Intramural Res Program, NIH, Baltimore, MD 21224 USA

[2] NIA, Res Resources Branch, Intramural Res Program, NIH, Baltimore, MD 21224 USA

[3] Univ Navarra, CIMA, Dept Oncol, Pamplona 31008, Spain

[4] Methodist Hosp, Dept Syst Med & Bioengn, Res Inst, Houston, TX 77030 USA

来源：

MOLECULAR CELL | 2012年 / 47卷 / 04期

关键词：

BINDING PROTEIN HUR; LONG NONCODING RNAS; EXPRESSION; REPRESSION; DISEASE;

D O I：

10.1016/j.molcel.2012.06.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mammalian long intergenic noncoding RNAs (lincRNAs) are best known for modulating transcription. Here we report a posttranscriptional function for lincRNA-p21 as a modulator of translation. Association of the RNA-binding protein HuR with lincRNA-p21 favored the recruitment of let-7/Ago2 to lincRNA-p21, leading to lower lincRNA-p21 stability. Under reduced HuR levels, lincRNA-p21 accumulated in human cervical carcinoma He La cells, increasing its association with JUNB and CTNNB1 mRNAs and selectively lowering their translation. With elevated HuR, lincRNA-p21 levels declined, which in turn derepressed JunB and beta-catenin translation and increased the levels of these proteins. We propose that HuR controls translation of a subset of target mRNAs by influencing lincRNA-p21 levels. Our findings uncover a role for lincRNA as a posttranscriptional inhibitor of translation.

引用

页码：648 / 655

页数：8

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