Primer on Agar-Based Microbial Imaging Mass Spectrometry

被引：120

作者：

Yang, Jane Y. ^{[1
]}

Phelan, Vanessa V. ^{[2
]}

Simkovsky, Ryan ^{[4
]}

Watrous, Jeramie D. ^{[1
]}

Trial, Rachelle M.

Fleming, Tinya C.

Wenter, Roland ^{[5
]}

Moore, Bradley S. ^{[2
,5
]}

Golden, Susan S. ^{[4
]}

Pogliano, Kit

Dorrestein, Pieter C. ^{[1
,2
,3
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Ctr Chronobiol, Div Biol Sci, La Jolla, CA 92093 USA

[5] Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA

来源：

JOURNAL OF BACTERIOLOGY | 2012年 / 194卷 / 22期

基金：

美国国家卫生研究院;

关键词：

BACILLUS-SUBTILIS; PSEUDOMONAS-AERUGINOSA; METABOLIC EXCHANGE; SEQUENCE-ANALYSIS; GENE CLUSTERS; BACTERIAL; IDENTIFICATION; BIOSYNTHESIS; COMMUNICATION; ANNOTATION;

D O I：

10.1128/JB.00823-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) imaging mass spectrometry (IMS) applied directly to microbes on agar-based medium captures global information about microbial molecules, allowing for direct correlation of chemotypes to phenotypes. This tool was developed to investigate metabolic exchange factors of intraspecies, interspecies, and polymicrobial interactions. Based on our experience of the thousands of images we have generated in the laboratory, we present five steps of microbial IMS: culturing, matrix application, dehydration of the sample, data acquisition, and data analysis/interpretation. We also address the common challenges encountered during sample preparation, matrix selection and application, and sample adherence to the MALDI target plate. With the practical guidelines described herein, microbial IMS use can be extended to bio-based agricultural, biofuel, diagnostic, and therapeutic discovery applications.

引用

页码：6023 / 6028

页数：6

共 71 条

[1] STUDIES ON CHARACTERIZATION OF LIPOPOLYSACCHARIDES FROM 2 STRAINS OF SERRATIA MARCESCENS [J].

ALAUPOVIC, P ;

OLSON, AC ;

TSANG, J .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1966, 133 (A2) :546-+

[2] SBSPKS: structure based sequence analysis of polyketide synthases [J].

Anand, Swadha ;

Prasad, M. V. R. ;

Yadav, Gitanjali ;

Kumar, Narendra ;

Shehara, Jyoti ;

Ansari, Md. Zeeshan ;

Mohanty, Debasisa .

NUCLEIC ACIDS RESEARCH, 2010, 38 :W487-W496

[3] NRPS-PKS: a knowledge-based resource for analysis of NRPS/PKS megasynthases [J].

Ansari, MZ ;

Yadav, G ;

Gokhale, RS ;

Mohanty, D .

NUCLEIC ACIDS RESEARCH, 2004, 32 :W405-W413

[4] Quorum sensing in bacterial virulence [J].

Antunes, L. Caetano M. ;

Ferreira, Rosana B. R. ;

Buckner, Michelle M. C. ;

Finlay, B. Brett .

MICROBIOLOGY-SGM, 2010, 156 :2271-2282

[5] SURFACTIN A CRYSTALLINE PEPTIDELIPID SURFACTANT PRODUCED BY BACILLUS SUBTILIS - ISOLATION CHARACTERIZATION AND ITS INHIBITION OF FIBRIN CLOT FORMATION [J].

ARIMA, K ;

KAKINUMA, A ;

TAMURA, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 31 (03) :488-&

[6] Bacterially speaking [J].

Bassler, BL ;

Losick, R .

CELL, 2006, 125 (02) :237-246

[7] Identification of a Novel Two-Peptide Lantibiotic, Lichenicidin, following Rational Genome Mining for LanM Proteins [J].

Begley, Maire ;

Cotter, Paul D. ;

Hill, Colin ;

Ross, R. Paul .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (17) :5451-5460

[8] Dealing with the Unknown: Metabolomics and Metabolite Atlases [J].

Bowen, Benjamin P. ;

Northen, Trent R. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2010, 21 (09) :1471-1476

[9] Fruiting body formation by Bacillus subtilis [J].

Branda, SS ;

González-Pastor, JE ;

Ben-Yehuda, S ;

Losick, R ;

Kolter, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) :11621-11626

[10] EXPRESSION OF THE PSBDII GENE IN SYNECHOCOCCUS SP STRAIN-PCC-7942 REQUIRES SEQUENCES DOWNSTREAM OF THE TRANSCRIPTION START SITE [J].

BUSTOS, SA ;

GOLDEN, SS .

JOURNAL OF BACTERIOLOGY, 1991, 173 (23) :7525-7533

← 1 2 3 4 5 6 7 8 →