Primer on Agar-Based Microbial Imaging Mass Spectrometry

被引：120

作者：

Yang, Jane Y. ^{[1
]}

Phelan, Vanessa V. ^{[2
]}

Simkovsky, Ryan ^{[4
]}

Watrous, Jeramie D. ^{[1
]}

Trial, Rachelle M.

Fleming, Tinya C.

Wenter, Roland ^{[5
]}

Moore, Bradley S. ^{[2
,5
]}

Golden, Susan S. ^{[4
]}

Pogliano, Kit

Dorrestein, Pieter C. ^{[1
,2
,3
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Ctr Chronobiol, Div Biol Sci, La Jolla, CA 92093 USA

[5] Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA

来源：

JOURNAL OF BACTERIOLOGY | 2012年 / 194卷 / 22期

基金：

美国国家卫生研究院;

关键词：

BACILLUS-SUBTILIS; PSEUDOMONAS-AERUGINOSA; METABOLIC EXCHANGE; SEQUENCE-ANALYSIS; GENE CLUSTERS; BACTERIAL; IDENTIFICATION; BIOSYNTHESIS; COMMUNICATION; ANNOTATION;

D O I：

10.1128/JB.00823-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) imaging mass spectrometry (IMS) applied directly to microbes on agar-based medium captures global information about microbial molecules, allowing for direct correlation of chemotypes to phenotypes. This tool was developed to investigate metabolic exchange factors of intraspecies, interspecies, and polymicrobial interactions. Based on our experience of the thousands of images we have generated in the laboratory, we present five steps of microbial IMS: culturing, matrix application, dehydration of the sample, data acquisition, and data analysis/interpretation. We also address the common challenges encountered during sample preparation, matrix selection and application, and sample adherence to the MALDI target plate. With the practical guidelines described herein, microbial IMS use can be extended to bio-based agricultural, biofuel, diagnostic, and therapeutic discovery applications.

引用

页码：6023 / 6028

页数：6

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