A Guide to the Perplexed on the Specificity of Antibodies

被引：122

作者：

Saper, Clifford B. ^{[1
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机构：

[1] Beth Israel Deaconess Med Ctr, Dept Neurol, Boston, MA 02215 USA

[2] Harvard Univ, Sch Med, Dept Neurol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Program Neurosci, Boston, MA 02115 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2009年 / 57卷 / 01期

关键词：

immunohistochemistry; immunocytochemistry; controls;

D O I：

10.1369/jhc.2008.952770

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation. (J Histochem Cytochem 57:1-5, 2009)

引用

页码：1 / 5

页数：5

共 6 条

[1]

COONS A H, 1958, Gen Cytochem Methods, V1, P399

[2]

FORMALDEHYDE FIXATION [J].