Improved high performance liquid chromatographic determination of ginsenosides in Panax ginseng-based pharmaceuticals using a diol column

被引:22
作者
Bonfill, M
Casals, I
Palazón, J
Mallol, A
Morales, C
机构
[1] Univ Barcelona, Fac Farm, Lab Fisiol Vegetal, E-08028 Barcelona, Spain
[2] Univ Barcelona, Serveis Cientificotecn, E-08028 Barcelona, Catalonia, Spain
关键词
D O I
10.1002/bmc.120.abs
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A reversed-phase high-performance liquid chromatographic assay for the simultaneous quantitative determination of seven ginsenosides, Rb-1, Rb-2, Rc, Rd, Rg(1), Re and Rf in pharmaceutical preparations is described. Chromatographic separation was achieved in less than 20 min using a 250 x 4 mm Lichrospher, 5 pm, 100 A diol column with detection at 203 nm. The method was validated over the range of 2.5-20 ng/muL using a 20 muL sample volume. The average accuracy at five concentrations was 90-100%, and the within-day and between-day precision ranged from 1 to 7% expressed as coefficient of variation. The detection limit and the quantitation limit of the method were 20 and 50 ng injected for each ginsenoside, respectively. Copyright (C) 2002 John Wiley Sons, Ltd.
引用
收藏
页码:68 / 72
页数:5
相关论文
共 11 条
[1]   DETERMINATION OF GINSENOSIDES IN GINSENG CRUDE EXTRACTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
CHUANG, WC ;
SHEU, SJ .
JOURNAL OF CHROMATOGRAPHY A, 1994, 685 (02) :243-251
[2]   STUDIES ON PLANT-TISSUE CULTURES .39. SAPONIN PRODUCTION IN CELL-SUSPENSION CULTURES OF PANAX GINSENG [J].
FURUYA, T ;
YOSHIKAWA, T ;
ORIHARA, Y ;
ODA, H .
PLANTA MEDICA, 1983, 48 (02) :83-87
[3]  
INOMATA S, 1993, PLANT CELL REP, V12, P681, DOI 10.1007/BF00233419
[4]   PREPARATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON CHEMICALLY MODIFIED POROUS-GLASS - ISOLATION OF ACIDIC SAPONINS FROM GINSENG [J].
KANAZAWA, H ;
NAGATA, Y ;
MATSUSHIMA, Y ;
TOMODA, M ;
TAKAI, N .
JOURNAL OF CHROMATOGRAPHY, 1991, 537 (1-2) :469-474
[5]  
Nandhasri Pranee, 1993, ASEAN Food Journal, V8, P159
[6]  
Park MK, 1996, J CHROMATOGR A, V736, P77
[7]   IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF GINSENOSIDES IN PANAX GINSENG EXTRACTS AND PRODUCTS [J].
PIETTA, P ;
MAURI, P ;
RAVA, A .
JOURNAL OF CHROMATOGRAPHY, 1986, 356 (01) :212-219
[8]  
SAMUKAWA K, 1995, CHEM PHARM BULL, V43, P137, DOI 10.1248/cpb.43.137
[9]   ANALYTICAL METHODS VALIDATION - BIOAVAILABILITY, BIOEQUIVALENCE, AND PHARMACOKINETIC STUDIES [J].
SHAH, VP ;
MIDHA, KK ;
DIGHE, S ;
MCGILVERAY, IJ ;
SKELLY, JP ;
YACOBI, A ;
LAYLOFF, T ;
VISWANATHAN, CT ;
COOK, CE ;
MCDOWALL, RD ;
PITTMAN, KA ;
SPECTOR, S .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1992, 81 (03) :309-312
[10]  
SHIBATA S, 1985, J EC MED PLANT RES, P217