Phosphorylation of a novel zinc-finger-like protein, ZPR9, by murine protein serine/threonine kinase 38 (MPK38)

被引:43
作者
Seong, HA
Gil, M
Kim, KT
Kim, SJ
Ha, H
机构
[1] Chungbuk Natl Univ, Res Ctr Bioresource & Hlth, Sch Life Sci, Dept Biochem, Cheongju 361763, Chongbuk, South Korea
[2] Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, South Korea
[3] Kyung Hee Univ, Sch Dent, Dept Pharmacol, Seoul 130701, South Korea
关键词
association in vivo; subcellular localization; substrate in vivo; yeast two-hybrid screen;
D O I
10.1042/0264-6021:3610597
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have identified previously a new murine protein serine/threonine kinase, MPK38, closely related to the sucrose-non-fermenting protein kinase family [Gil, Yang, Lee, Choi and Ha (1997) Gene 195, 295-301]. Using the C-terminal half of the putative human counterpart of MPK38, HPK38, as a bait in a yeast two-hybrid screen of a human HeLa cDNA library, it was discovered that the zinc-finger-motif-containing protein, termed zinc-finger-like protein 9 (ZPR9), bound both HPK38 and MPK38. In a co-expression assay, ZPR9 associated with MPK38 in vivo, and we showed that the ZPR9 is also phosphorylated by MPK38. In addition, ZPR9 physically interacts with itself in mammalian cells. The ZPR9 cDNA hybridized with a mRNA species of approx. 1.7 kb in Northern-blot analysis. The ZPR9 transcript was detected in all tissues examined, including lung, kidney, spleen, liver and brain. Co-expression of ZPR9 with MPK38 caused the accumulation of ZPR9 in the nucleus. These findings suggest a potentially important role for ZPR9 in MPK38-mediated signal transduction, and that ZPR9 is a physiological substrate of MPK38 in vivo.
引用
收藏
页码:597 / 604
页数:8
相关论文
共 34 条
[11]   Cloning and expression of a cDNA encoding a novel protein serine/threonine kinase predominantly expressed in hematopoietic cells [J].
Gil, M ;
Yang, Y ;
Lee, Y ;
Choi, I ;
Ha, H .
GENE, 1997, 195 (02) :295-301
[12]   THE PROTEIN-KINASE FAMILY - CONSERVED FEATURES AND DEDUCED PHYLOGENY OF THE CATALYTIC DOMAINS [J].
HANKS, SK ;
QUINN, AM ;
HUNTER, T .
SCIENCE, 1988, 241 (4861) :42-52
[13]  
Heyer BS, 1997, MOL REPROD DEV, V47, P148, DOI 10.1002/(SICI)1098-2795(199706)47:2&lt
[14]  
148::AID-MRD4&gt
[15]  
3.0.CO
[16]  
2-M
[17]  
Heyer BS, 1999, DEV DYNAM, V215, P344
[18]   TRANSCRIPTIONAL REGULATION BY EXTRACELLULAR SIGNALS - MECHANISMS AND SPECIFICITY [J].
HILL, CS ;
TREISMAN, R .
CELL, 1995, 80 (02) :199-211
[19]  
HU HM, 1994, J BIOL CHEM, V269, P30069
[20]   THE REGULATION OF TRANSCRIPTION BY PHOSPHORYLATION [J].
HUNTER, T ;
KARIN, M .
CELL, 1992, 70 (03) :375-387