Resistance against antimicrobial peptides is independent of Escherichia coli AcrAB, Pseudomonas aeruginosa MexAB and Staphylococcus aureus NorA efflux pumps

被引：37

作者：

Rieg, Siegbert ^{[1
]}

Huth, Anja ^{[1
]}

Kalbacher, Hubert ^{[2
]}

Kern, Winfried V. ^{[1
]}

机构：

[1] Univ Hosp, Dept Med, Ctr Infect Dis & Travel Med, D-79106 Freiburg, Germany

[2] Univ Tubingen, Med & Nat Sci Res Ctr, Tubingen, Germany

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2009年 / 33卷 / 02期

关键词：

Innate immunity; Antimicrobial peptides; Resistance mechanisms; Efflux pumps; FLUOROQUINOLONE RESISTANCE; RND-TYPE; PROTEIN;

D O I：

10.1016/j.ijantimicag.2008.07.032

中图分类号：

R51 [传染病];

学科分类号：

100201 [内科学];

摘要：

The role of clinically important multidrug resistance (MDR) efflux pumps in bacterial resistance to various human antimicrobial peptides (AMPs), including cathelicidin LL-37, the alpha-defensins human neutrophil peptides (HNPs)-1-3 and HD-5 and the beta-defensins hBD-2 and -3, was investigated. AMP susceptibility testing was performed by killing assays and standard minimal inhibitory concentration assays. AMP susceptibility was determined in Escherichia coli and Pseudomonas aeruginosa strains overexpressing resistance-nodulation-cell division (RND)-type pumps AcrAB and MexAB, respectively, and in their pump-deficient parental strains. Furthermore, the impact of a member of the major facilitator (MF) efflux pump family was investigated in Staphylococcus aureus overexpressing NorA and in wild-type strains. The E. coli AcrAB and P. aeruginosa MexAB RND-type efflux pumps as well as the S. aureus NorA MF efflux pump were unable to confer resistance to AMPs. These findings do not support a critical role of MDR efflux pumps in the tested pathogens as a strategy to increase virulence by circumventing the antimicrobial action of innate defence AMPs. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：174 / 176

页数：3

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