Carbohydrate analysis prepares to enter the "omics" era

被引:10
作者
Bidlingmaier, S [1 ]
Snyder, M [1 ]
机构
[1] Yale Univ, New Haven, CT 06520 USA
来源
CHEMISTRY & BIOLOGY | 2002年 / 9卷 / 04期
关键词
D O I
10.1016/S1074-5521(02)00133-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this issue, Houseman and Mrksich describe a carbohydrate array preparation method that can be used to analyze protein-carbohydrate interactions and to characterize the substrate specificity of a carbohydrate-modifying enzyme. Carbohydrate chips were prepared by a novel procedure that allows the covalent attachment of carbohydrate-diene conjugates to a specially engineered monolayer surface. The surface presents a precisely controllable ratio of reactive benzoquinone and inert ethylene glycol groups. Nonspecific adsorption of proteins to the surface is extremely low, and the surface is compatible with popular detection techniques. The immobilization technique was demonstrated to be compatible with recently developed automated solid phase carbohydrate synthesis methods, paving the way for the development of highly complex carbohydrate arrays.
引用
收藏
页码:400 / 401
页数:2
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