QUANTITATIVE MONITORING OF GENE-EXPRESSION PATTERNS WITH A COMPLEMENTARY-DNA MICROARRAY

被引：6378

作者：

SCHENA, M

SHALON, D

DAVIS, RW

BROWN, PO

机构：

[1] STANFORD UNIV, MED CTR, BECKMAN CTR, DEPT BIOCHEM, STANFORD, CA 94305 USA

[2] STANFORD UNIV, MED CTR, BECKMAN CTR, HOWARD HUGHES MED INST, STANFORD, CA 94305 USA

来源：

SCIENCE | 1995年 / 270卷 / 5235期

关键词：

D O I：

10.1126/science.270.5235.467

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.

引用

页码：467 / 470

页数：4

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