Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries

被引：196

作者：

Antinori, Monica ^{[1
]}

Licata, Emanuele ^{[1
]}

Dani, Gianluca ^{[1
]}

Ceerusico, Fabrizio ^{[1
]}

Versaci, Caterina ^{[1
]}

Antinori, Severino ^{[1
]}

机构：

[1] RAPRUI Day Hosp, Rome, Italy

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2007年 / 14卷 / 01期

关键词：

cryopreservation; Cryotop; human oocytes; pregnancy rate; survival rate; vitrification;

D O I：

10.1016/S1472-6483(10)60766-3

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Vitrification, an ultra-rapid cooling technique, offers a new perspective in attempts to develop an optimal cryopreservation procedure for human oocytes and embryos. To further evaluate this method for human oocytes, 796 mature oocytes (metaphase 11) were collected from 120 volunteers. Since Italian legislation allows the fertilization of a maximum of only three oocytes per woman, there were 463 supernumerary oocytes; instead of being discarded, they were vitrified. When, in subsequent cycles, these oocytes were utilized, 328 out of 330 (99.4%) oocytes survived the warming procedure. The fertilization rate, pregnancy rate and implantation rate per embryo were 92.9, 32.5 and 13.2% respectively. Thus, as already reported in the literature, the vitrification procedure seems to be highly effective, safe (since healthy babies have been born) and easy to apply. In situations where embryo cryopreservation is not permitted (as in Italy), there is now good indication for routine application of the method, once further standardization is achieved.

引用

页码：72 / 79

页数：8

共 55 条

[21] High survival rate of vitrified human oocytes results in clinical pregnancy [J].