Click chemistry to construct fluorescent oligonucleotides for DNA sequencing

被引：221

作者：

Seo, TS

Li, ZM

Ruparel, H

Ju, JY

机构：

[1] Columbia Univ Coll Phys & Surg, Columbia Genome Ctr, New York, NY 10032 USA

[2] Columbia Univ, Dept Chem Engn, New York, NY 10027 USA

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2003年 / 68卷 / 02期

关键词：

D O I：

10.1021/jo026615r

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Click chemistry 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with singlebase resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.

引用

页码：609 / 612

页数：4

共 34 条

[31] Mainchain pseudopolyrotaxanes via post-threading with cucurbituril [J].