The direct quantitative correspondence between theoretical predictions and the measured plasma fluctuations and transport is tested by performing nonlinear gyrokinetic simulations with the GS2 code. This is a continuation of previous work with gyrofluid simulations [D. W. Ross , Phys. Plasmas 9, 177 (2002)], and the same L-mode reference discharge in the DIII-D tokamak [J. L. Luxon and L. G. Davis, Fusion Technol. 8, 441 (1985)] is studied. The simulated turbulence is dominated by ion temperature gradient (ITG) modes, corrected by trapped-electron, passing-electron and impurity effects. The energy fluxes obtained in the gyrokinetic simulations are comparable to, even somewhat higher than, those of the earlier work, and the simulated ion thermal transport, corrected for ExB flow shear, exceeds the experimental value by more than a factor of 2. The simulation also overestimates the density fluctuation level. Varying the local temperature gradient shows a stiff response in the flux and an apparent up-shift from the linear mode threshold [A. M. Dimits , Phys. Plasmas 7, 969 (2000)]. This effect is insufficient, within the estimated error, to bring the results into conformity with the experiment. (C) 2002 American Institute of Physics.