Lineages of acidophilic archaea revealed by community genomic analysis

被引：160

作者：

Baker, Brett J.

Tyson, Gene W.

Webb, Richard I.

Flanagan, Judith

Hugenholtz, Philip

Allen, Eric E.

Banfield, Jillian F. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Earth & Planetary Sci, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Berkeley, CA 94720 USA

[3] Univ Queensland, Ctr Microscopy & Microanal, Brisbane, Qld 4072, Australia

[4] Univ Queensland, Dept Microbiol & Parasitol, Brisbane, Qld 4072, Australia

来源：

SCIENCE | 2006年 / 314卷 / 5807期

关键词：

D O I：

10.1126/science.1132690

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Novel, low-abundance microbial species can be easily overlooked in standard polymerase chain reaction (PCR)-based surveys. We used community genomic data obtained without PCR or cultivation to reconstruct DNA fragments bearing unusual 16S ribosomal RNA ( rRNA) and protein-coding genes from organisms belonging to novel archaeal lineages. The organisms are minor components of all biofilms growing in pH 0.5 to 1.5 solutions within the Richmond Mine, California. Probes specific for 16S rRNA showed that the fraction less than 0.45 micrometers in diameter is dominated by these organisms. Transmission electron microscope images revealed that the cells are pleomorphic with unusual folded membrane protrusions and have apparent volumes of < 0.006 cubic micrometer.

引用

收藏

页码：1933 / 1935

页数：3

相关论文

共 24 条

[1]

Related assemblages of sulphate-reducing bacteria associated with ultradeep gold mines of South Africa and deep basalt aquifers of Washington State [J].

Baker, BJ ;

Moser, DP ;

MacGregor, BJ ;

Fishbain, S ;

Wagner, M ;

Fry, NK ;

Jackson, B ;

Speolstra, N ;

Loos, S ;

Takai, K ;

Lollar, BS ;

Fredrickson, J ;

Balkwill, D ;

Onstott, TC ;

Wimpee, CF ;

Stahl, DA .

ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (04) :267-277

[2]

Extremely acidophilic protists from acid mine drainage host Rickettsiales-lineage endosymbionts that have intervening sequences in their 16S rRNA genes [J].

Baker, BJ ;

Hugenholtz, P ;

Dawson, SC ;

Banfield, JF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (09) :5512-5518

[3]

Microbial communities in acid mine drainage [J].

Baker, BJ ;

Banfield, JF .

FEMS MICROBIOLOGY ECOLOGY, 2003, 44 (02) :139-152

[4]

Bacterial rhodopsin:: Evidence for a new type of phototrophy in the sea [J].

Béjà, O ;

Aravind, L ;

Koonin, EV ;

Suzuki, MT ;

Hadd, A ;

Nguyen, LP ;

Jovanovich, S ;

Gates, CM ;

Feldman, RA ;

Spudich, JL ;

Spudich, EN ;

DeLong, EF .

SCIENCE, 2000, 289 (5486) :1902-1906

[5]

Phylogeny of microorganisms populating a thick, subaerial, predominantly lithotrophic biofilm at an extreme acid mine drainage site [J].

Bond, PL ;

Smriga, SP ;

Banfield, JF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (09) :3842-3849

[6]

Community genomics among stratified microbial assemblages in the ocean's interior [J].

DeLong, EF ;

Preston, CM ;

Mincer, T ;

Rich, V ;

Hallam, SJ ;

Frigaard, NU ;

Martinez, A ;

Sullivan, MB ;

Edwards, R ;

Brito, BR ;

Chisholm, SW ;

Karl, DM .

SCIENCE, 2006, 311 (5760) :496-503

[7]

Acid mine drainage biogeochemistry at Iron Mountain, California [J].

Druschel, GK ;

Baker, BJ ;

Gihring, TM ;

Banfield, JF .

GEOCHEMICAL TRANSACTIONS, 2004, 5 (02) :13-32

[8]

Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean [J].

Francis, CA ;

Roberts, KJ ;

Beman, JM ;

Santoro, AE ;

Oakley, BB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (41) :14683-14688

[9]

Genome streamlining in a cosmopolitan oceanic bacterium [J].

Giovannoni, SJ ;

Tripp, HJ ;

Givan, S ;

Podar, M ;

Vergin, KL ;

Baptista, D ;

Bibbs, L ;

Eads, J ;

Richardson, TH ;

Noordewier, M ;

Rappé, MS ;

Short, JM ;

Carrington, JC ;

Mathur, EJ .

SCIENCE, 2005, 309 (5738) :1242-1245

[10]

A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont [J].

Huber, H ;

Hohn, MJ ;

Rachel, R ;

Fuchs, T ;

Wimmer, VC ;

Stetter, KO .

NATURE, 2002, 417 (6884) :63-67