2dx -: User-friendly image processing for 2D crystals

被引：139

作者：

Gipson, Bryant ^{[1
]}

Zeng, Xiangyan ^{[1
]}

Zhang, Zi Yan ^{[1
]}

Stahlberg, Henning ^{[1
]}

机构：

[1] Univ Calif Davis, Davis, CA 95616 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2007年 / 157卷 / 01期

关键词：

2dx software; electron crystallography; 2D crystals; membrane protein; structure determination; computer image processing; MRC software;

D O I：

10.1016/j.jsb.2006.07.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron crystallography determines the structure of two-dimensional (2D) membrane protein crystals and other 2D crystal systems. Cryotransmission electron microscopy records high-resolution electron micrographs, which require computer processing for three-dimensional structure reconstruction. We present anew software system 2dx, which is designed as a user-friendly, platform-independent software package for electron crystallography. 2dx assists in the management of an image-processing project, guides the user through the processing of 2D crystal images, and provides transparence for processing tasks and results. Algorithims are implemented in the form of script templates reminiscent of c-shell scripts. These templates can be easily modified or replaced by the user and can also execute modular stand-alone programs from the MRC software or from other image processing software packages. 2dx is available under the GNU General Public License at 2dx.org. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：64 / 72

页数：9

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[1] Projection structure of the human copper transporter CTR1 at 6-A resolution reveals a compact trimer with a novel channel-like architecture [J].