Redundancy and specialization among plant microRNAs:: role of the MIR164 family in developmental robustness

被引:282
作者
Sieber, Patrick
Wellmer, Frank
Gheyselinck, Jacqueline
Riechmann, Jose Luis
Meyerowitz, Elliot M. [1 ]
机构
[1] CALTECH, Div Biol, Pasadena, CA 91125 USA
[2] Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland
来源
DEVELOPMENT | 2007年 / 134卷 / 06期
关键词
microRNA; miR164; organogenesis; developmental robustness; phyllotaxis; CUP-SHAPED COTYLEDON; SHOOT APICAL MERISTEM; CUP-SHAPED-COTYLEDON; ARABIDOPSIS-THALIANA; FLOWER DEVELOPMENT; GENE-EXPRESSION; LEAF POLARITY; TARGET GENES; RNA PATHWAYS; EVOLUTION; DUPLICATION;
D O I
10.1242/dev.02817
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In plants, members of microRNA ( miRNA) families are often predicted to target the same or overlapping sets of genes. It has thus been hypothesized that these miRNAs may act in a functionally redundant manner. This hypothesis is tested here by studying the effects of elimination of all three members of the MIR164 family from Arabidopsis. It was found that a loss of miR164 activity leads to a severe disruption of shoot development, in contrast to the effect of mutation in any single MIR164 gene. This indicates that these miRNAs are indeed functionally redundant. Differences in the expression patterns of the individual MIR164 genes imply, however, that redundancy among them is not complete, and that these miRNAs show functional specialization. Furthermore, the results of molecular and genetic analyses of miR164-mediated target regulation indicate that miR164 miRNAs function to control the transcript levels, as well as the expression patterns, of their targets, suggesting that they might contribute to developmental robustness. For two of the miR164 targets, namely CUP-SHAPED COTYLEDON1 (CUC1) and CUC2, we provide evidence for their involvement in the regulation of growth and show that their derepression in miR164 loss-of-function mutants is likely to account for most of the mutant phenotype.
引用
收藏
页码:1051 / 1060
页数:10
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