Vinculin gene is non-essential in Drosophila melanogaster

被引：54

作者：

Alatortsev, VE

Kramerova, IA

Frolov, MV

Lavrov, SA

Westphal, ED

机构：

[1] Institute of Molecular Genetics, Russ. Acad. Sci., Kurchatov Sq. 46

来源：

FEBS LETTERS | 1997年 / 413卷 / 02期

关键词：

protein homology; vinculin function; chromosome rearrangement;

D O I：

10.1016/S0014-5793(97)00901-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vinculin is thought to be an important cytoskeletal protein in the linkage between actin cytoskeleton and integrin transmembrane receptors. We identified Vinculin (Vinc) gene in the X chromosome of D. melanogaster, Drosophila vinculin is highly homologous in its N- and C-terminal domains both to mammalian and nematode vinculins, and contains internal repeats and proline-rich region typical for vinculins. The X chromosome rearrangement In(1LR)pn2a was found to disrupt Vinc so that the coding sequence is interrupted by the (AAGAG)(n) satellite DNA. Northern analysis revealed that the Vinc transcript is completely absent in the In(1LR)pn2a homozygous flies, Surprisingly, these Vinc flies are viable and fertile. This finding highlights plasticity and adaptive capacity of cellular cytoskeletal and anchorage system. (C) 1997 Federation of European Biochemical Societies.

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页码：197 / 201

页数：5

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