Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique

被引:161
作者
Choi, HK
Choi, YH
Verberne, M
Lefeber, AWM
Erkelens, C
Verpoorte, R
机构
[1] Leiden Univ, Inst Biol, Metab Sect, Dept Pharmacognosy, NL-2300 RA Leiden, Netherlands
[2] Chung Ang Univ, Coll Pharm, Seoul 156756, South Korea
[3] Gorlaeus Labs, Inst Chem, Div NMR, NL-2300 RA Leiden, Netherlands
关键词
metabolomic analysis; tobacco; Nicotiana tabacum; H-1; NMR; multivariate analysis; salicylic acid;
D O I
10.1016/j.phytochem.2004.01.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The metabolomic analysis of wild type and constitutive salicylic acid producing tobacco plants (CSA tobacco, Nicotiana tabacum 'Samsun' NN) plants overexpressing salicylate biosynthetic genes was carried out by H-1 NMR spectrometry and multivariate analysis techniques. The principle component analysis (PCA) of the H-1 NMR spectra showed a clear discrimination between those samples by PC1 and PC2. The discrimination of non-inoculated, TMV-virus inoculated, and systemic leaves or veins could also be obtained by PCA analysis. Major peaks in H-1 NMR spectra contributing to the discrimination were assigned as those of chlorogenic acid, malic acid, and Sugars. This method allows an efficient differentiation between wild type and transgenic plants without any pre-purification steps. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:857 / 864
页数:8
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