Differential metabolic profiling by HPLC-PDA-MS of wild type and transgenic tobacco plants constitutively producing salicylic acid

被引：2

作者：

Halim, VA ^{[1
]}

Verberne, MC ^{[1
]}

Verpoorte, R ^{[1
]}

机构：

[1] Leiden Univ, Leiden Amsterdam Ctr Drug Res, Div Pharacognosy, NL-2300 RA Leiden, Netherlands

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 03期

关键词：

metabolic profiling; tobacco; transgenic plants; salicylic acid overexpression;

D O I：

10.1081/JLC-120017176

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The metabolite profiles of wild type tobacco plants and transgenic tobacco plants, constitutively producing salicylic acid (SA), were compared by means of liquid chromatography with online photo diode array and mass spectrometer detector (LC-PDA-MS). Statistical analysis of the peak area of 15 major peaks showed significant differences in quantities for three compounds, which are present in the extract before acid hydrolysis, containing mainly aglucones. By comparison of the retention time, UV spectra, and molecular weight with reference compounds, two of these peaks were identified as chlorogenic acid and rutin. The extracts obtained after acidic hydrolysis did not show any significant difference.

引用

页码：369 / 383

页数：15

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