Subunit organization of the membrane-bound HIV-1 envelope glycoprotein trimer

被引：125

作者：

Mao, Youdong ^{[1
]}

Wang, Liping ^{[1
]}

Gu, Christopher ^{[1
]}

Herschhorn, Alon ^{[1
]}

Xiang, Shi-Hua ^{[1
]}

Haim, Hillel ^{[1
]}

Yang, Xinzhen ^{[2
]}

Sodroski, Joseph ^{[1
,3
,4
]}

机构：

[1] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Microbiol & Immunobiol,Dept Canc Immunol & A, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Dept Med,Div Viral Pathogenesis, Boston, MA 02115 USA

[3] Massachusetts Gen Hosp, MIT, Ragon Inst, Boston, MA 02114 USA

[4] Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2012年 / 19卷 / 09期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

IMMUNODEFICIENCY-VIRUS TYPE-1; NEUTRALIZATION EPITOPE; CONFORMATIONAL-CHANGES; VARIABLE LOOPS; AIDS PATIENTS; CD4; BINDING; GP120; CORE; HTLV-III; GP41; ANTIBODY;

D O I：

10.1038/nsmb.2351

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The trimeric human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) spike is a molecular machine that mediates virus entry into host cells and is the sole target for virus-neutralizing antibodies. The mature Env spike results from cleavage of a trimeric glycoprotein precursor, gp160, into three gp120 and three gp41 subunits. Here, we describe an similar to 11-angstrom cryo-EM structure of the trimeric HIV-1 Env precursor in its unliganded state. The three gp120 and three gp41 subunits form a cage-like structure with an interior void surrounding the trimer axis. Interprotomer contacts are limited to the gp41 transmembrane region, the torus-like gp41 ectodomain and a trimer-association domain of gp120 composed of the V1, V2 and V3 variable regions. The cage-like architecture, which is unique among characterized viral envelope proteins, restricts antibody access, reflecting requirements imposed by HIV-1 persistence in the host.

引用

页码：893 / 899

页数：7

共 54 条

[1] MAJOR GLYCOPROTEIN ANTIGENS THAT INDUCE ANTIBODIES IN AIDS PATIENTS ARE ENCODED BY HTLV-III [J].