共 36 条
Structure of Monomeric Yeast and Mammalian Sec61 Complexes Interacting with the Translating Ribosome
被引:226
作者:
Becker, Thomas
[2
,3
]
Bhushan, Shashi
[2
,3
]
Jarasch, Alexander
[2
,3
]
Armache, Jean-Paul
[2
,3
]
Funes, Soledad
[2
,3
,4
]
Jossinet, Fabrice
[5
]
Gumbart, James
[6
]
Mielke, Thorsten
[7
,8
]
Berninghausen, Otto
[2
,3
]
Schulten, Klaus
[6
]
Westhof, Eric
[5
]
Gilmore, Reid
[1
]
Mandon, Elisabet C.
[1
]
Beckmann, Roland
[2
,3
]
机构:
[1] Univ Massachusetts, Sch Med, Dept Mol Pharmacol & Biochem, Worcester, MA 01605 USA
[2] Univ Munich, Gene Ctr Munich, D-81377 Munich, Germany
[3] Univ Munich, Ctr Integrated Prot Sci, Dept Chem & Biochem, D-81377 Munich, Germany
[4] Univ Nacl Autonoma Mexico, Dept Bioquim, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
[5] Univ Strasbourg, CNRS, Inst Biol Mol & Cellulaire, Architecture & React ARN, F-67084 Strasbourg, France
[6] Univ Illinois, Beckman Inst, Dept Phys, Urbana, IL 61801 USA
[7] Max Planck Inst Mol Genet, Ultrastrukturnetzwerk, D-14195 Berlin, Germany
[8] Charite, Inst Med Phys & Biophys, D-10117 Berlin, Germany
来源:
关键词:
SIGNAL RECOGNITION PARTICLE;
ENDOPLASMIC-RETICULUM;
PROTEIN-TRANSLOCATION;
SEQUENCE RECOGNITION;
CHANNEL COMPLEX;
BINDING;
MEMBRANE;
SECYEG;
ARCHITECTURE;
RECEPTOR;
D O I:
10.1126/science.1178535
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation.
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页码:1369 / 1373
页数:5
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