Co-culture of arbuscular mycorrhiza-like fungi (Piriformospora indica and Sebacina vermifera) with plant cells of Linum album for enhanced production of podophyllotoxins:: a first report

被引：74

作者：

Baldi, Ashish ^{[1
]}

Jain, Abhishek ^{[1
]}

Gupta, Nishant ^{[1
]}

Srivastava, A. K. ^{[1
]}

Bisaria, V. S. ^{[1
]}

机构：

[1] Indian Inst Technol Delhi, Plant Cell Culture Lab, Dept Biochem Engn & Biotechnol, New Delhi 110016, India

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 09期

关键词：

co-culture; Linum album; Piriformospora indica; plant/fungi interactions; podophyllotoxin; Sebacina vermifera;

D O I：

10.1007/s10529-008-9736-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and 1.9 mg/l, respectively after 12 d cultivation. Co-cultures of L. album cells with axenically cultivable arbuscular mycorrhiza-like fungi, Piriformospora indica and Sebacina vermifera, were established for the first time. These enhanced podophyllotoxin and 6-methoxypodophyllotoxin production by about four- and eight-fold, respectively, along with a 20% increase in biomass compared to the control cultures.

引用

页码：1671 / 1677

页数：7

共 27 条

[1]

BALDI A, 2007, MED PLANT BIOTECHNOL, P117

[2]

Piriformospora indica and Sebacina vermifera increase growth performance at the expense of herbivore resistance in Nicotiana attenuata [J].