Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin

被引：30

作者：

Chattopadhyay, S

Srivastava, AK

Bhojwani, SS

Bisaria, VS

机构：

[1] Indian Inst Technol, Dept Biochem Engn & Biotechnol, New Delhi 110016, India

[2] Univ Delhi, Dept Bot, New Delhi 110003, India

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 24期

关键词：

podophyllotoxin; Podophyllum hexandrum; suspension culture;

D O I：

10.1023/A:1013704116860

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A suspension culture of Podophyllum hexandrum was established. As the cultures grew, reduction in cell viability, biomass and product yield were associated with browning of culture medium, clumping of cells and drop in medium pH. Supplementation of the medium with both polyvinylpyrrollidone (PVP) and pectinase eliminated these problems. PVP at 10 g l(-1) was optimum for both growth of and product formation in P. hexandrum suspension cultures.

引用

页码：2063 / 2066

页数：4

共 13 条

[1]

SOMATIC EMBRYOGENESIS IN TISSUE-CULTURES OF PODOPHYLLUM-HEXANDRUM [J].