trans-Acting proteins involved in RNA encapsidation and viral assembly in human immunodeficiency virus type 1

被引:34
作者
Kaye, JF [1 ]
Lever, AML [1 ]
机构
[1] ADDENBROOKES HOSP,DEPT MED,CAMBRIDGE CB2 2QQ,ENGLAND
关键词
D O I
10.1128/JVI.70.2.880-886.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The human immunodeficiency virus type 1 gag gene product Pr55(gag) self-assembles when expressed on its own in a variety of eukaryotic systems. Assembly in T lymphocytes has not previously been studied, nor is it clear whether Pr55(gag) particles can package genomic RNA or if the Gag-Pol polyprotein is required. We have used a series of constructs that express Gag or Gag-Pol proteins with or without the viral protease in transient transfections in COS-1 cells and also expressed stably in CD4(+) T cells to study this. Deletion of the p6 domain at the C terminus of protease-negative pr55(gag) did not abolish particle release, while truncation of the nucleocapsid protein reduced it significantly, particularly in lymphocytes. Gag-Pol polyprotein was released from T cells in the absence of pr55(gag) but did not encapsidate RNA. Pr55(gag) encapsidated human immunodeficiency virus type 1 RNA whether expressed in a protease-positive or protease-negative context. p6 was dispensable for RNA encapsidation. Marked differences in the level of RNA export were noted between the different cell lines.
引用
收藏
页码:880 / 886
页数:7
相关论文
共 62 条
[1]   IDENTIFICATION OF A SIGNAL IN A MURINE RETROVIRUS THAT IS SUFFICIENT FOR PACKAGING OF NONRETROVIRAL RNA INTO VIRIONS [J].
ADAM, MA ;
MILLER, AD .
JOURNAL OF VIROLOGY, 1988, 62 (10) :3802-3806
[2]   MUTATIONS OF RNA AND PROTEIN SEQUENCES INVOLVED IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PACKAGING RESULT IN PRODUCTION OF NONINFECTIOUS VIRUS [J].
ALDOVINI, A ;
YOUNG, RA .
JOURNAL OF VIROLOGY, 1990, 64 (05) :1920-1926
[3]   TRANSACTIVATION OF THE MINUS-STRAND DNA TRANSFER BY NUCLEOCAPSID PROTEIN DURING REVERSE TRANSCRIPTION OF THE RETROVIRAL GENOME [J].
ALLAIN, B ;
LAPADATTAPOLSKY, M ;
BERLIOZ, C ;
DARLIX, JL .
EMBO JOURNAL, 1994, 13 (04) :973-981
[4]   AVIAN RETROVIRAL RNA ENCAPSIDATION - REEXAMINATION OF FUNCTIONAL 5' RNA SEQUENCES AND THE ROLE OF NUCLEOCAPSID CYS-HIS MOTIFS [J].
ARONOFF, R ;
HAJJAR, AM ;
LINIAL, ML .
JOURNAL OF VIROLOGY, 1993, 67 (01) :178-188
[5]   FUNCTIONAL SITES IN THE 5' REGION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA FORM DEFINED STRUCTURAL DOMAINS [J].
BAUDIN, F ;
MARQUET, R ;
ISEL, C ;
DARLIX, JL ;
EHRESMANN, B ;
EHRESMANN, C .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 229 (02) :382-397
[6]   EVIDENCE THAT THE PACKAGING SIGNAL OF MOLONEY MURINE LEUKEMIA-VIRUS EXTENDS INTO THE GAG REGION [J].
BENDER, MA ;
PALMER, TD ;
GELINAS, RE ;
MILLER, AD .
JOURNAL OF VIROLOGY, 1987, 61 (05) :1639-1646
[7]   POTENTIAL METAL-BINDING DOMAINS IN NUCLEIC-ACID BINDING-PROTEINS [J].
BERG, JM .
SCIENCE, 1986, 232 (4749) :485-487
[8]   SPECIFIC BINDING OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GAG POLYPROTEIN AND NUCLEOCAPSID PROTEIN TO VIRAL RNAS DETECTED BY RNA MOBILITY SHIFT ASSAYS [J].
BERKOWITZ, RD ;
LUBAN, J ;
GOFF, SP .
JOURNAL OF VIROLOGY, 1993, 67 (12) :7190-7200
[9]   MYRISTOYLATION-DEPENDENT REPLICATION AND ASSEMBLY OF HUMAN IMMUNODEFICIENCY VIRUS-1 [J].
BRYANT, M ;
RATNER, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) :523-527
[10]  
BUKRINSKAYA AG, 1993, MOL BIOL+, V27, P22