Apparent minimum free energy requirements for methanogenic Archaea and sulfate-reducing bacteria in an anoxic marine sediment

Among the most fundamental constraints governing the distribution of micro organisms in the environment is the availability of chemical energy at biologically useful levels. To assess the minimum free energy yield that can support microbial metabolism in situ, we examined the thermodynamics of H-2-consuming processes in anoxic sediments from Cape Lookout Bight, NC, USA. Depth distributions of H-2 partial pressure, along with a suite of relevant concentration data, were determined in sediment cores collected in November (at 14.5 degreesC) and August (at 27 degreesC) and used to calculate free energy yields for methanogenesis and sulfate reduction. At both times of year, and for both processes, free energy yields gradually decreased (became less negative) with depth before reaching an apparent asymptote. Sulfate-reducing bacteria exhibited an asymptote of -19.1 +/-1.7 kJ (mol SO42-)(-1), while methanogenic Archaea were apparently supported by energy yields as small as -10.6 +/-0.7 kJ (mol CH4)(-1). (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.