Effect of cisplatin and c-myb antisense phosphorothioate oligodeoxynucleotides combination on a human colon carcinoma cell line in vitro and in vivo

被引：24

作者：

DelBufalo, D

Cucco, C

Leonetti, C

Citro, G

DAgnano, I

Benassi, M

Geiser, T

Zon, G

Calabretta, B

Zupi, G

机构：

[1] REGINA ELENA INST CANC RES,LAB EXPT CHEMOTHERAPY,I-00161 ROME,ITALY

[2] THOMAS JEFFERSON UNIV,JEFFERSON CANC INST,PHILADELPHIA,PA 19107

[3] CNR,INST BIOMED TECHNOL,I-00185 ROME,ITALY

[4] REGINA ELENA INST CANC RES,LAB MED PHYS & EXPERT SYST,I-00161 ROME,ITALY

[5] LYNX THERAPEUT,HAYWARD,CA 94545

来源：

BRITISH JOURNAL OF CANCER | 1996年 / 74卷 / 03期

关键词：

phosphorothioate oligodeoxynucleotide; c-myb; cisplatin; colon carcinoma; LoVo Dr;

D O I：

10.1038/bjc.1996.370

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We investigated the effect of c-myb antisense phosphorothioate oligodeoxynucleotides [(S)ODNs] and cisplatin (CDDP) combination on the human colon carcinoma cell line LoVo Dx both in vitro and in nude mice bearing LoVo Dx solid tumour. We show that antisense (S)ODN treatment decreases c-myb mRNA and protein expression, induces growth arrest in the G(I) phase of the cell cycle, and inhibits cell proliferation. In vivo treatment with c-myb antisense (S)ODNs results in a reduction in tumour growth. A greater inhibition of cell proliferation in vitro and a higher increase of tumour growth inhibition and growth delay in vivo were obtained with the combination of (S)ODNs and CDDP than when the two agents were administered separately. This comparative study, using the same tumour cell line in vitro and in vivo, suggests that c-myb antisense (S)ODNs might be useful in the therapy of colon cancer in combination with antineoplastic drugs.

引用

页码：387 / 393

页数：7