Naringin and bone marrow mesenchymal stem cells repair articular cartilage defects in rabbit knees through the transforming growth factor-β superfamily signaling pathway

被引：24

作者：

Ye, Chao ^{[1
]}

Chen, Jing ^{[2
]}

Qu, Yi ^{[1
]}

Liu, Hang ^{[3
]}

Yan, Junxing ^{[4
]}

Lu, Yingdong ^{[5
]}

Yang, Zheng ^{[6
]}

Wang, Fengxian ^{[1
]}

Li, Pengyang ^{[1
]}

机构：

[1] Beijing Univ Chinese Med, Dongzhimen Hosp, Dept Orthoped, 5 Haiyuncang St, Beijing 100700, Peoples R China

[2] Beijing Univ Chinese Med, Affiliated Hosp 3, Preventat Treatment Dis Dept, Beijing 100029, Peoples R China

[3] Beijing Univ Chinese Med, Huguosi Hosp, Dept Orthoped, Beijing 100035, Peoples R China

[4] Tongzhou Dist Hosp Integrated Tradit Chinese Med, Dept Orthoped, Beijing 101100, Peoples R China

[5] China Acad Chinese Med Sci, Guanganmen Hosp, Dept Pathol, Beijing 100053, Peoples R China

[6] Beijing Univ Chinese Med, SATCM Key Lab Renowned Phys & Class Formula, Beijing 100029, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2020年 / 20卷 / 05期

关键词：

naringin; rabbit; knee joint; cartilage defect; bone mesenchymal stem cells; transforming growth factor-beta; superfamily signaling pathway; TGF-BETA; NATURAL-PRODUCTS; SOX9; CHONDROCYTES; CHONDROGENESIS; OSTEOARTHRITIS; MECHANISMS; THERAPY; DIFFERENTIATION; MICROFRACTURE;

D O I：

10.3892/etm.2020.9187

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

The present study aimed to assess the effect of a combination of naringin and rabbit bone marrow mesenchymal stem cells (BMSCs) on the repair of cartilage defects in rabbit knee joints and to assess possible involvement of the transforming growth factor-beta (TGF-beta) signaling pathway in this process. After establishing an articular cartilage defect model in rabbit knees, 20 New Zealand rabbits were divided into a sham operation group (Sham), a model group (Mod), a naringin treatment group (Nar), a BMSC group (BMSCs) and a naringin + BMSC group (Nar/BMSCs). At 12 weeks after treatment, the cartilage was evaluated using the International Cartilage Repair Society (ICRS)'s macroscopic evaluation of cartilage repair scale, the ICRS's visual histological assessment scale, the Modified O'Driscoll grading system, histological staining (hematoxylin and eosin staining, toluidine blue staining and safranin O staining) and immunohistochemical staining (type-II collagen, TGF-beta 3 and SOX-9 immunostaining). Using the above grading systems to quantify the extent of repair, histological quantification and macro quantification of joint tissue repair showed that the Nar/BMSCs group displayed repair after treatment in comparison to the untreated Mod group. Among the injury model groups (Mod, Nar, BMSCs and Nar/BMSCs), the Nar/BMSCs group displayed the highest degree of morphological repair. The results of histological and immunohistochemical staining of the repaired region of the joint defect indicated that the BMSCs had a satisfactory effect on the repair of the joint structure but had a poor effect on the repair of cartilage quality. The Nar/BMSCs group displayed satisfactory therapeutic effects on both repair of the joint structure and cartilage quality. The expression level of type-II collagen was high in the Nar/BMSCs group. Additionally, staining of TGF-beta 3 and SOX-9 in the Nar/BMSCs group was the strongest compared with that of any other group in the present study. Naringin and/BMSCs together demonstrated a more efficient repair effect on articular cartilage defects in rabbit knees than the use of either treatment alone in terms of joint structure and cartilage quality. One potential mechanism of naringin action may be through activation and continuous regulation of the TGF-beta superfamily signaling pathway, which can promote BMSCs to differentiate into chondrocytes.

引用

页数：13