A European field strain of bluetongue virus derived from two parental vaccine strains by genome segment reassortment

被引：116

作者：

Batten, Carrie A. ^{[1
]}

Maan, Sushila ^{[1
]}

Shaw, Andrew E. ^{[1
]}

Maan, Narender S. ^{[1
]}

Mertens, Peter P. C. ^{[1
]}

机构：

[1] Inst Anim Hlth, Arbovirus Res Grp, Pirbright GU24 0NF, England

来源：

VIRUS RESEARCH | 2008年 / 137卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

bluetongue; BTV; orbivirus; dsRNA; reassortment; BTV vaccine;

D O I：

10.1016/j.virusres.2008.05.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Since 1998, nine bluetongue virus (BTV) strains from serotypes 1, 2. 4, 8, 9 and 16 have invaded Europe, killing >2 million animals (mainly sheep). Live vaccine strains of BTV-2, 4, 9 and 16 have also been used in the region. The BTV genome is composed of ten linear-segments of dsRNA, and events in Europe have provided opportunities for different strains to exchange/reassort genome segments, generating novel progeny-vi ruses. Genome segment 2 (Seg-2) encodes outer capsid protein VP2, the primary determinant of virus serotype, while Seg-5 encodes NS1, which forms 'tubules' within the cell-cytoplasm. Seg-2 and Seg-5 from 15 European isolates, and vaccine/reference strains, of BTV-2 and BTV-16, were sequenced. Isolates from the same serotype showed >92% nt identity in Seg-5, but <84% identity between types. However, published data for Seg-5 of BTV-16 from Italy 2002 showed <83% nt identity with other BTV-16 strains, but was identical to the BTV-2 vaccine strain (used in Italy during 2002, and annually in a multivalent vaccine in Israel since 1995) indicating that ITL2002 is a reassortant between the BTV-2 and BTV-16 vaccine strains. This represents the first detection of a reassortant BTV strain within Europe, highlighting concerns about the use of live BTV vaccines in the region. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：56 / 63

页数：8

共 61 条

[21] High throughput detection of bluetongue virus by a new real-time fluorogenic reverse transcription-polymerase chain reaction:: Application on clinical samples from current Mediterranean outbreaks [J].

Jiménez-Clavero, MA ;

Agüero, M ;

Miguel, ES ;

Mayoral, T ;

López, MC ;

Ruano, MJ ;

Romero, E ;

Monaco, F ;

Polci, A ;

Savini, G ;

Gómez-Tejedor, C .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2006, 18 (01) :7-17

[22]

KATZ JB, 1993, AM J VET RES, V54, P2021

[23] MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment [J].

Kumar, S ;

Tamura, K ;

Nei, M .

BRIEFINGS IN BIOINFORMATICS, 2004, 5 (02) :150-163

[24] Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes [J].

Maan, S. ;

Maan, N. S. ;

Samuel, A. R. ;

Rao, S. ;

Attoui, H. ;

Mertens, P. P. C. .

JOURNAL OF GENERAL VIROLOGY, 2007, 88 :621-630

[25]

Maan S, 2004, Vet Ital, V40, P489

[26]

Maan S., 2004, THESIS U LONDON

[27] Sequence analysis of bluetongue virus serotype 8 from the Netherlands 2006 and comparison to other European strains [J].

Maan, Sushila ;

Maan, Narender S. ;

Ross-Smith, Natalie ;

Batten, Carrie A. ;

Shaw, Andrew E. ;

Anthony, Simon J. ;

Samuel, Alan R. ;

Darpel, Karin E. ;

Veronesi, Eva ;

Oura, Chris A. L. ;

Singh, Karam P. ;

Nomikou, Kyriaki ;

Potgieter, Abraham C. ;

Attoui, Houssam ;

van Rooij, Eugene ;

van Rijn, Piet ;

De Clercq, Kris ;

Vandenbussche, Frank ;

Zientara, Stephan ;

Breard, Emmanuel ;

Sailleau, Corinne ;

Beer, Martin ;

Hoffman, Bernd ;

Mellor, Philip S. ;

Mertens, Peter P. C. .

VIROLOGY, 2008, 377 (02) :308-318

[28] Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses [J].

Maan, Sushila ;

Rao, Shujing ;

Maan, Narender Singh ;

Anthony, Simon John ;

Attoui, Houssam ;

Samuel, Alan Richard ;

Paul, Peter ;

Mertens, Clement .

JOURNAL OF VIROLOGICAL METHODS, 2007, 143 (02) :132-139

[29] VARIATION AMONGST THE NEUTRALIZING EPITOPES OF BLUETONGUE VIRUSES ISOLATED IN THE UNITED-STATES IN 1979-1981 [J].

MACLACHLAN, NJ ;

ROSSITTO, PV ;

HEIDNER, HW ;

IEZZI, LG ;

YILMA, TD ;

DEMAULA, CD ;

OSBURN, BI .

VETERINARY MICROBIOLOGY, 1992, 31 (04) :303-316

[30] PHYLOGENETIC CHARACTERIZATION OF BLUETONGUE VIRUSES FROM NATURALLY-INFECTED INSECTS, CATTLE AND SHEEP IN AUSTRALIA [J].

MCCOLL, KA ;

GOULD, AR ;

PRITCHARD, LI ;

MELVILLE, L ;

BELLIS, G .

AUSTRALIAN VETERINARY JOURNAL, 1994, 71 (04) :102-105

← 1 2 3 4 5 6 7 →