Differential effects of chemotherapeutic agents on the Bcl-2/Bax apoptosis pathway in human breast cancer cell line MCF-7

被引：86

作者：

Leung, LK ^{[1
]}

Wang, TTY ^{[1
]}

机构：

[1] NCI, Basic Res Lab, Frederick Canc Res & Dev Ctr, NIH, Frederick, MD 21702 USA

来源：

BREAST CANCER RESEARCH AND TREATMENT | 1999年 / 55卷 / 01期

关键词：

apoptosis; Bax; Bcl-2; breast; chemotherapy; estradiol;

D O I：

10.1023/A:1006190802590

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The present study explored the effects of three commonly used chemotherapeutic agents on the Bcl-2/Bax apoptosis pathway and the interaction of these chemotherapeutic drugs with the estradiol-mediated regulation of this pathway. Our results showed that: (1) Treatment of MCF-7 cells with Adriamycin resulted in time- and concentration-dependent decreases in Bcl-2 and increases in Bax mRNA and protein levels. (2) Camptothecin elicited similar trends on Bcl-2 and Bax as Adriamycin, while etoposide, at 50-100 fold (1-5 mu M) the effective concentration of Adriamycin and camptothecin, only resulted in an increase in Bax mRNA levels. (3) Adriamycin and camptothecin, but not etoposide, were effective in suppressing estradiol-stimulated increases in Bcl-2 mRNA levels. Our study provides evidence that the Bcl-2/Bax apoptosis pathway may be differentially regulated by chemotherapeutic agents. In addition, interaction between these agents and estradiol on the Bcl-2/Bax apoptosis pathway may also exist.

引用

页码：73 / 83

页数：11

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