Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy

被引：31

作者：

Ashjian, P

Elbarbary, A

Zuk, P

DeUgarte, DA

Benhaim, P

Marcu, L

Hedrick, MH

机构：

[1] Cedars Sinai Med Ctr, Dept Surg, Biophoton Res & Technol Dev Lab, MIST Lab, Los Angeles, CA 90048 USA

[2] Univ Calif Los Angeles, Sch Med, Dept Surg, Lab Regenerat Bioengn & Repair, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Sch Med, Dept Orthoped, Los Angeles, CA 90095 USA

[4] Univ So Calif, Dept Biomed, Los Angeles, CA 90089 USA

[5] Univ So Calif, Dept Elect Engn, Los Angeles, CA 90089 USA

来源：

TISSUE ENGINEERING | 2004年 / 10卷 / 3-4期

关键词：

D O I：

10.1089/107632704323061771

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.

引用

页码：411 / 420

页数：10