Direct synthesis of well-defined heterotelechelic polymers for bioconjugations

被引：132

作者：

Boyer, Cyrille ^{[1
]}

Liu, Jingquan ^{[1
]}

Bulmus, Volga ^{[1
]}

Davis, Thomas P. ^{[1
]}

Barner-Kowollik, Christopher ^{[1
]}

Stenzel, Martina H. ^{[1
]}

机构：

[1] Univ New S Wales, Sch Chem Sci & Engn, CAMD, Sydney, NSW 2052, Australia

来源：

MACROMOLECULES | 2008年 / 41卷 / 15期

关键词：

D O I：

10.1021/ma800289u

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Direct synthesis of well-defined heterotelechelic polymers having functional groups allowing the chemoselective bioconjugations would be desirable to enhance the versatility of polymers for bioconjugations and bio-related applications. Considering this, well-defined alpha-azide, omega-dithiopyridine polymers were synthesized in one step via the reversible addition-fragmentation chain transfer (RAFT) polymerization. The telechelic functionality (i.e., mole ratio of the omega-dithiopyridine to the alpha-azide end group) of polymers was above 0.90, indicating the efficient generation of well-defined heterotelechelic polymers. The heterotelechelic functionality for chemoselective bioconjugations was tested by reacting alpha-azide, omega-dithiopyridine poly(NIPAAm) with model biomolecules, i.e., biotin/avidin, glutathione, and bovine serum albumin, via click and thiol-disulfide exchange chemistries. Near-stoichiometric conjugation with biomolecules indicated high functionality of the polymer end groups.

引用

页码：5641 / 5650

页数：10

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